Daria Pašalić
Department of Medical Chemistry, Biochemistry and Clinical Chemistry
Zagreb University School of Medicine
Šalata ul 2.
10 000 Zagreb, Croatia
Phone +385 (1) 4590 205; +385 (1) 4566 940
E-mail: dariapasalic [at] gmail [dot] com

Useful links

P02-1 (Oral presentation)

Jadrić R1, Hasić S1, Kiseljaković E1, Ćorić J2, Huskić J1, Winterhalter-Jadrić M1. PO2-1: Influence of ferritin and CRP concentration on atherogenic index. Biochemia Medica 2009;19(Suppl 1):S108.
1Institute for Physiology and Biochemistry, Medical Faculty Sarajevo, Sarajevo, Bosnia and Herzegovina
2Institute for Clinical Chemistry and Biochemistry, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Corresponding author:rjadric [at] hotmail [dot] com
Introduction: We have investigated ferritin and C-reactive protein levels and their relation as cardiovascular disease risk markers.
Material and methods: Patients were blood donors, divided in groups by age and gender, and pregnant women as a control. Blood samples were collected by venepunction, and assayed for ferritin and CRP level determination, with atherogenic index calculated from patient’s lipid profile.
Results: We obtained lower feritin values in fertile women subgroup, with significant differences between male and female groups of same age, as well as when compared to women in menopause. Ferritin levels were not significantly linked with CRP concentration, but patients with high ferritin were more likely to have high CRP concentration. At the same time, all patients with high CRP had higher atherogenic index.
Conclusion: Our results are emphasizing the importance of ferritin and CRP concentration monitoring, as parameters influencing future cardiovascular events.
P02-2 (Oral presentation)
Horvat V1, Jurić S1, Butković-Soldo S1, Mandić D2, Mandić S1, Majetić-Cetina N1. PO2-2: Protein S100 determination in acute cerebrovascular insult. Biochemia Medica 2009;19(Suppl 1):S108-S109.
1Department for Clinical Laboratory Diagnostics, Osijek University Hospital, Osijek, Croatia
2Institute of Public Health for Osijek-Baranya County, Osijek, Croatia
Corresponding author:phorvat [dot] vesna [at] gmail [dot] com
Introduction: S100 protein is acidic calcium-binding protein which is main structural component of cytosol, mainly astroglia cells. Structural cerebral damage leads to releasing of that protein in cerebrospinal fluid and bloodstream. Aim of this preliminary prospective study was to investigate value of early determination of serum S-100 protein in acute ICV.
Materials and methods: 35 patients (14 men and 21 women), aged 58-88 years, admitted to Neurology Clinic of Clinical Hospital Osijek with symptoms of acute ICV were investigated. Within 24 hours from onset of symptoms on all patients we performed brain CT and collected blood sample. S100 protein concentration was measured using ECLIA method on Elecsys 2010 (ROCHE) system for immunochemical analyses. 33 subjects (15 men and 18 women), aged 45-81 years, who were not neurological patients were chosen for control group. We compared S100 concentration in acute ICV patient with those in control group. Results were analyzed with Mann-Whitney Rank Sum test.
Results: Patients with acute ischemic ICV had statistically significant higher median of S100 serum values (P = 0.002), compared to S100 values of control group, but lower (P = 0.003) compared to patients with acute hemorrhagic ICV. Early brain CT confirmed ischemic ICV only on minority of patients.
Conclusion: Considering that early ischemic changes in brain are rarely noticeable on CT within first 24 hours from onset of symptoms, it seems that determination of serum S100 could be useful in acute ischemic ICV diagnostic.
Mladina B, Šupe Domić D, Stanišić L, Čepić K, Dujmov I. PO2-3: Correlation between concentration of myeloperoxidase with age and sex. Biochemia Medica 2009;19(Suppl 1):S109-S110.
Split University Hospital Center, Split, Croatia
Corresponding author:blanka [dot] mladina [at] kbsplit [dot] hr
Introduction: Myeloperoxidase (MPO) is a hemoprotein stored in azurophilic granules of polymorphonuclear neutrophils and macrophages and released into extracellular fluid in the setting of inflammatory process and promotes oxidative damage. MPO has been detected in atherosclerotic plaques and plasma MPO levels have been shown to be a predictor of major adverse cardiac events in patients presenting with chest pain to the emergency room. Therefore MPO is to be used in conjunction with clinical history, ECG and cardiac biomarkers to evaluate patients presenting with chest pain who are at risk for major adverse cardiac events, including myocardial infarction, need for revascularization or death.
Aim: The aim of this study was to test concentration of MPO in a healthy subject in the County of Split - Dalmatia and to find out if there is a correlation between the MPO concentration and subjects’ age and sex.
Patients and methods: The sample consisted of 73 healthy subjects, 33 male (aged from 29 to 81) and female (aged from 24 to 79). MPO concentration is measured in human EDTA plasma by chemiluminiscent microparticle immunoassay on Architect i1000SR (CMIA) company ABBOTT.
Results: The observed values for MPO concentration for women are 89.8 ± 89.6 pmol/L and for men 87.6 ± 74.9 pmol/L. Comparing MPO concentration with women’s age we have found correlation coefficient r = 0.07; P = 0.659, and with men’s age r = -0.26; P = 0.137.
Conclusion: Concentration for MPO that we have found in our laboratory is in narrower range from reference range of the manufacturer. It indicates that external factors as climate, nutrition, environmental conditions and life habits influence on the results. According to our results neither subjects’ sex nor ages are related to MPO concentration.
Hasic S, Jadrić R, Kiseljaković E, Winterhalter-Jadrić M. PO2-4: Biochemical markers of myocardial damage in rat model. Biochemia Medica 2009;19(Suppl 1):S110-S111.
Institute for Physiology and Biochemistry, Medical Faculty Sarajevo, Sarajevo, Bosnia and Herzegovina
Corresponding author:sabahetahasic [at] yahoo [dot] com
Introduction: This study was performed to determine whether cardiac troponin T (cTnT) and myoglobin could detect rat myocardial damage induced by isoproterenol (ISO) aplication.
Material and method: Twenty-four rats (N = 24) were divided into four groups: control (N = 8), ISO I group (N = 8), ISO II group (N = 8), and ISO III group (N = 8). ISO, single, i.p. dose 250 mg/kg was given to male, adult, albino, Wistar rats to induce myocardial damage. Saline was administered to rats of control group. Cardiac TnT and myoglobin were measured by ECLA sandwich immunoassay in rat sera of control group and 30, 60 and 120 minutes after ISO aplication. Obtained data were analysed by two-tail, unpaired Students’ t-test. Significance was set at 0.05.
Results: Results are reported as mean ± SD. Mean cTnT blood value was significantly increased in all ISO groups. Beetween ISO groups there were siginficant differences in cTnT blood level (P < 0.05). Significant increase of blood myoglobin value was in ISO III group in comparison with control (P < 0.05). Beetween ISO groups there were not significant differences in myoglobin blood level.
Conclusions: This study showed that applicated dose of ISO 250 mg/kg induced rat myocardial damage. Cardiac TnT is sensitive, specific biomarker of rat myocardial damage. Myoglobin showed poorer sensitivity probably trough its’ rapid kinetic and elimination trough kidneys.
Vogrinc Ž, Trbojević-Čepe M, Ljubić H, Sertić J. PO2-5: Leptin and small, dense LDL particles. Biochemia Medica 2009;19(Suppl 1):S111-S112.
Clinical Institute of Laboratory Diagnosis, Zagreb University School of Medicine and Clinical Hospital Center, Zagreb, Croatia
Corresponding author:zvogrinc [at] kbc-zagreb [dot] hr
Background: Leptin, a peptide hormone secreted by the adipose tissue is primarily involved in the regulation of food intake and energy expenditure. Plasma leptin concentration is proportional to body adiposity and is markedly increased in obese individuals. Leptin exerts many potentially atherogenic effects such as induction of endothelial dysfunction, stimulation of inflammatory reaction, oxidative stress, proliferation of vascular smooth muscle cells etc. We investigated the relationship between serum leptin levels and the occurrence of small dense LDL particles (pattern B) to evaluate the possible influence of this adipokine on LDL particle size distribution.
Materials and methods: We determined serum leptin concentrations in group of 52 dyslipidemic subjects with visceral adiposity and related metabolic syndrome, and in a group of 54 healthy blood donors with normal lipid profile and normal distribution of LDL particle size. Serum leptin concentrations were determined by comercially available ELISA test (Mediagnost, Germany). LDL particle size determination was performed by gradient polyacrylamide gel electrophoresis developed in our laboratory.
Results: Pathologic LDL phenotype (LDL phenotype B) was detected in 27/52 dyslipidemic patients, and the mean value of leptin concentrations in this selected subgroup of patients was 14.5 ug/L. The mean value of leptin concentrations in the whole group of dyslipidemic patient was 11.4 ug/L and in the group of controls it was 8.1 ug/L.
Conclusions: The higher levels of leptin in selected subgroup of patients with LDL phenotype B suggest that this adipokine coud be involved in mechanisms of the small, dense LDL particles production.
Coen Herak D1, Miloš M1, Leniček Krleža J2, Zrinski Topić R3, Dodig S3, Zadro R1. PO2-6: Association between C-reactive protein and arterial ischemic stroke in children. Biochemia Medica 2009;19(Suppl 1):S112-S113.
1Clinical Institute of Laboratory Diagnosis, Zagreb University School of Medicine and Clinical Hospital Center, Zagreb, Croatia
2Department of Laboratory Diagnosis, Children’s Hospital Zagreb, Zagreb, Croatia
3Department of Clinical Laboratory Diagnosis, Srebrnjak Children’s Hospital, Zagreb, Croatia
Corresponding author:desireecoen [at] yahoo [dot] com
Introduction: Increased concentrations of high-sensitivity C-reactive protein (hs-CRP) are associated with increased risk for atherothrombotic disease in adults, but limited data are available in children. This prompted us to investigate the possible association between hs-CRP concentrations and arterial ischemic stroke (AIS) in children.
Patients and methods: hs-CRP was measured in 41 children (25 boys, 16 girls) aged < 18 years, with an established diagnosis of AIS. Children were classified into 2 groups, childhood AIS (N = 21) and perinatal/neonatal AIS (N = 20). The control group consisted of 56 healthy children (36 boys, 20 girls) aged 2-17 years. CRP was measured by using particle-enhanced immunonephelometry (CardioPhase hsCRP, Siemens Medical Solutions Diagnostics).
Results: By using a cut-off value of 0.84 mg/L (90th percentile), significantly higher frequency of elevated hs-CRP values (P = 0.014) was found in children with AIS (29.3%) than in healthy children (8.9%). A twofold higher median hs-CRP value was identified in childhood AIS (0.47 mg/L) compared to healthy children (0.22 mg/L), but without statistical significance (P = 0.154). Higher median hs-CRP value, although not statistically significant (P = 0.784), was found in childhood AIS (0.47 mg/L) compared to perinatal/neonatal AIS (0.24 mg/L). Moreover, an almost threefold higher median hs-CRP value was identified in boys than in girls with AIS (0.47 and 0.16 mg/L, respectively), while in healthy children this difference was less pronounced (boys: 0.26 mg/L, girls: 0.19 mg/L).
Conclusions: According to obtained results there is a trend towards increased hs-CRP values in childhood AIS. This was more pronounced in boys and could explain their predominance in childhood AIS.
Kačkov S1, Šimundić AM2, Nikolac N2, Bilušić M1. PO2-7: Relationship between metabolic disorders (hyperglycemia, dislipidemia) and hyperuricemia in general population. Biochemia Medica 2009;19(Suppl 1):S113-S114.
1Medical biochemistry laboratory, Policlinic Bonifarm, Zagreb, Croatia
2University Department of Chemistry, Sestre Milosrdnice University Hospital, Zagreb, Croatia
Corresponding author:skackov [at] bonifarm [dot] hr
Abstract: Uric acid is associated with several components of the metabolic syndrome, insulin resistance, microalbuminuria, non-alcoholic fatty liver disease, and some other. Hyperuricemia more frequently occurs in males and overweight persons. The aim of this study was to determine the prevalence of hyperuricemia and its relationship with fasting glucose concentrations and lipid status in general population.
Material and methods: This retrospective study included 6,476 subjects older than 18 years, consecutively referred to our laboratory for routine blood testing, between June 2007 and February 2009. The concentration of glucose, triglycerides, total cholesterol, HDL-cholesterol, LDL-cholesterol and uric acid was measured in blood samples collected after an overnight fast. Subjects were divided into subgroups with normal and increased concentrations of measured parameters. Cut-off values were according to the ATP III criteria for glucose, recommended values were used for concentration of lipids, and for uric acid we used the upper limit of reference range according to the harmonized values of the Croatian Chamber of Medical biochemists.
Results: Hyperuricemia prevalence was 13.9% and it was significantly higher in males, than in females (26% vs. 6%; P < 0.001). The study subjects with hyperuricemia were often found to have increased values of glucose (9.3% versus 3.7%; P < 0.001), triglycerides (46.9% versus 17.6%; P < 0.001), total cholesterol (69.6% versus 51.9%; P < 0.001), LDL-cholesterol (64.5% versus 46.4%; P < 0.001), HDL-cholesterol (24.3% versus 13%; P < 0.001) compared to subjects with serum uric acid levels within the reference range.

Conclusion: Hyperuricemia is associated with fasting glucose concentrations and dyslipidemia in general population.