Journal Information
Journal ID (publisher-id): BM
Journal ID (nlm-ta): Biochem Med
Title: Biochemia Medica
Abbreviated Title: Biochem. Med.
ISSN (print): 1330-0962
ISSN (electronic): 1846-7482
Publisher: Croatian Society of Medical Biochemistry and Laboratory Medicine
Article Information
Copyright: 2016, Croatian Society of Medical Biochemistry
Date received: 26 January 2016
Date accepted: 21 May 2016
Publication date (print and electronic): 10 June 2016
Volume: 26
Issue: 2
Pages: 279-284
Publisher ID: bm-26-279
DOI: 10.11613/BM.2016.033
Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact: a case report
Saša Anžej Doma[1]
Andreas Hillarp[2]
Tadej Pajič[1]
Dušan Andoljšek[1]
Peter Černelč[1]
Irena Preldžnik Zupan[1]
[1] Department of Hematology, University Medical Centre Ljubljana, Slovenia
[2] Department of Clinical Chemistry and Transfusion Medicine, Halland Hospital, Halmstad, Sweden; at the time of the investigation working at the Coagulation laboratory of Malmö Centre for Thrombosis and Haemostasis, Skane University Hospital, Malmö, Sweden
Author notes:
Corresponding author: sasa_anzej@yahoo.co.uk
Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.
Keywords: inhibitor; autoantibody; blood coagulation factor; enzyme-linked immunosorbent assay
