Journal Information
Journal ID (publisher-id): BM
Journal ID (nlm-ta): Biochem Med (Zagreb)
Title: Biochemia Medica
Abbreviated Title: Biochem. Med. (Zagreb)
ISSN (print): 1330-0962
ISSN (electronic): 1846-7482
Publisher: Croatian Society of Medical Biochemistry and Laboratory Medicine
Article Information
Copyright statement: ©Croatian Society of Medical Biochemistry and Laboratory Medicine.
Copyright: 2024, Croatian Society of Medical Biochemistry
License (open-access):
This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Date received: 22 August 2023
Date accepted: 06 December 2023
Publication date: 15 February 2024
Publication date: 15 February 2024
Volume: 34
Issue: 1
Electronic Location Identifier: 010705
Publisher ID: bm-34-1-010705
DOI: 10.11613/BM.2024.010705
Effects of reverse osmosis membrane replacement of pure water system on clinical chemistry and immunoassay in clinical laboratory
Shaocong Liang[1]
Huaxian Wu[1]
Jiayi Zhao[2]
Xuanjie Guo[1]
Yongjie Qiang[1]
Xin Zhao[1]
Meng Lan[1]
Chongquan Zhao[1]
Author notes:
[*] Corresponding author: zhangdongxin@smu.edu.cn
Author contributions
D Zhang and S Liang conceived the project. S Liang, H Wu, Y Qiang, X Zhao, M Lan and C Zhao performed the study. S Liang, H Wu, J Zhao, X Guo and D Zhang analyzed the results. S Liang and D Zhang wrote the manuscript.
• The effects of reverse osmosis membrane replacement on clinical chemistry and immunoassay were showed
• Reverse osmosis membrane replacement interferes triglyceride test
• Triglyceride interference by reverse osmosis membrane replacement may be due to glycerol carryover
• The data provide reference for management of triglyceride interference by reverse osmosis membrane replacement
Introduction
Reverse osmosis (RO) membrane, key component of water-purifying equipment, is often stored in protection fluid containing substances such as glycerol, which may contaminate the water at replacement. This study aims to explore the effects of RO membrane replacement on clinical chemistry and immunoassay, particularly triglyceride (TG), providing reference for managing test interference caused by RO membrane replacement.
Materials and methods
The RO membrane of water-purifying equipment A, which provided water to C16000 biochemistry analyzer (Abbott Laboratories, Abbott Park, USA) and E801 electrochemiluminescence analyzer (Roche, Basel, Switzerland), was replaced. Water resistivity was recorded, and quality control (QC) tests were performed on C16000 and E801. Moreover, TG was measured in 29 of selected serum samples on C16000 at 0.5h and 10.5h after RO membrane replacement and on reference biochemistry analyzer BS2000M (Mindray Biomedical Electronics Co., Shenzhen, China), which was connected to water-purifying equipment B without RO membrane replacement. Finally, blank, calibrator 1 and calibrator 2 of TG reagent were measured on C16000 before and at 0.5h, 2.5h and 10.5h after RO membrane replacement. All statistical analyses of data were done using GraphPad Prism (GraphPad Software Inc., San Diego, USA), and a value of P < 0.05 was considered statistically significant.
Results
After RO membrane replacement, all QC results of clinical chemistry and immune tests passed except TG that showed positive bias of 536% and 371% at two levels, respectively. Moreover, TG results of the same serum samples were significantly higher at 0.5h than 10.5h after RO membrane replacement. Meanwhile, there was worse agreement and correlation of TG results between C16000 and BS2000M at 0.5h than 10.5h after replacement. Furthermore, the absorbance of TG blank, calibrator 1 and calibrator 2 was significantly higher at 0.5h and 2.5h after replacement than before replacement, and the absorbance gradually returned to normal value at 10.5h after replacement.
Conclusions
Replacement of RO membrane could cause significant interference to TG test while have no effects on other laboratory tests performed in the study, which may be due to glycerol contamination. Our data provides important reference for management of test interference caused by RO membrane replacement. Clinical laboratory should observe the effects of RO membrane replacement on laboratory tests through both water quality monitoring and QC detection.
Keywords: water purification; glycerol; clinical chemistry tests; immunoassay; triglycerides
