Journal Information
Journal ID (publisher-id): BM
Journal ID (nlm-ta): Biochem Med (Zagreb)
Title: Biochemia Medica
Abbreviated Title: Biochem. Med. (Zagreb)
ISSN (print): 1330-0962
ISSN (electronic): 1846-7482
Publisher: Croatian Society of Medical Biochemistry and Laboratory Medicine
Article Information
Copyright: 2017, Croatian Society of Medical Biochemistry
License (open-access):
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Date received: 26 April 2017
Date accepted: 22 July 2017
Publication date (electronic): 28 August 2017
Publication date (print): 15 October 2017
Volume: 27
Issue: 3
Electronic Location Identifier: 030901
Publisher ID: bm-27-3-030901
DOI: 10.11613/BM.2017.030901
The new Greiner FC-Mix tubes equal the old Terumo ones and are useful as glucose stabilizer after prolonged storage of samples
Graziella Bonetti[1]
Mariarosa Carta[2]
Author notes:
on behalf of the Italian joint SIBioC-SIPMeL Study Group on Diabetes Mellitus
Corresponding author: graziella.bonetti@asst-spedalicivili.it
Introduction
The aim of our study is to compare new Greiner tubes containing granulated citrate buffer with the Terumo ones and to verify if they are suitable for glucose stabilization after prolonged storage.
Materials and methods
In Study 1, blood was collected in two Terumo and two Greiner tubes from 40 healthy volunteers. Samples were stored at room temperature (RT) for 1 and 2 hours, respectively. Comparison was made by Deming regression. In Study 2, glucose was measured in a reference tube (N = 50), according to the ADA-NACB guidelines and in aliquots of Greiner samples maintained un-centrifuged at RT for 1, 2, 4 (N = 50) and 24, 48, 72 hours (N = 35).
Results
There were insignificant mixed biases between the Terumo and Greiner tubes. Compared to reference (5.3 mmol/L), glucose concentration in the new tubes was 5.4 (P < 0.05), 5.4 (P < 0.05), 5.3 (P = 0.265), 5.2 (P = 0.156), 5.3 (P < 0.05) and 5.2 (P < 0.05) mmol/L after 1, 2, 4, 24, 48 and 72 hours at RT, respectively. There was no biological difference between any of the time points up to 48 h (bias < ± 1.95%).
Keywords: diabetes; glucose; acidification; pre-analytical phase
